Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse kidney tissue, HepG2 cells, HEK-293 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IHC | See 4 publications below |
| IF | See 1 publications below |
Product Information
13122-1-AP targets ACADSB in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3851 Product name: Recombinant human ACADSB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 131-432 aa of BC013756 Sequence: VDASVAVFCEIQNTLINTLIRKHGTEEQKATYLPQLTTEKVGSFCLSEAGAGSDSFALKTRADKEGDYYVLNGSKMWISSAEHAGLFLVMANVDPTIGYKGITSFLVDRDTPGLHIGKPENKLGLRASSTCPLTFENVKVPEANILGQIGHGYKYAIGSLNEGRIGIAAQMLGLAQGCFDYTIPYIKERIQFGKRLFDFQGLQHQVAHVATQLEAARLLTYNAARLLEAGKPFIKEASMAKYYASEIAGQTTSKCIEWMGGVGYTKDYPVEKYFRDAKIGTIYEGASNIQLNTIAKHIDAEY 相同性解析による交差性が予測される生物種 |
| Full Name | acyl-Coenzyme A dehydrogenase, short/branched chain |
| Calculated molecular weight | 432 aa, 47 kDa |
| Observed molecular weight | 45-47 kDa |
| GenBank accession number | BC013756 |
| Gene Symbol | ACADSB |
| Gene ID (NCBI) | 36 |
| RRID | AB_10638904 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P45954 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ACADSB antibody 13122-1-AP | Download protocol |
| WB protocol for ACADSB antibody 13122-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Global mitochondrial protein import proteomics reveal distinct regulation by translation and translocation machinery | ||
Cell Mol Life Sci Mitochondrial FAD shortage in SLC25A32 deficiency affects folate-mediated one-carbon metabolism. | ||
Front Oncol Decreased Expression of ACADSB Predicts Poor Prognosis in Clear Cell Renal Cell Carcinoma. | ||
Cell Biol Int ACADSB regulates ferroptosis and affects the migration, invasion, and proliferation of colorectal cancer cells. | ||
Nat Commun AMPK induces degradation of the transcriptional repressor PROX1 impairing branched amino acid metabolism and tumourigenesis | ||
Phytother Res Icariside II prevents kidney fibrosis development in chronic kidney disease by promoting fatty acid oxidation |
