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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACADS Polyclonal antibody (16623-1-AP)

    WB analysis using 16623-1-AP (same clone as 16623-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16623-1-AP (ACADS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16623-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COS-7 cells using ACADS Polyclonal antibody (16623-1-AP)

    WB analysis of COS-7 using 16623-1-AP (same clone as 16623-1-PBS)

    COS-7 cells were subjected to SDS PAGE followed by western blot with 16623-1-AP (ACADS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16623-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using ACADS Polyclonal antibody (16623-1-AP)

    IP experiment of HEK-293 using 16623-1-AP (same clone as 16623-1-PBS)

    IP result of anti-ACADS (IP:16623-1-AP, 4ug; Detection:16623-1-AP 1:500) with HEK-293 cells lysate 3000ug. This data was developed using the same antibody clone with 16623-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ACADS Polyclonal antibody (16623-1-AP)

    IHC staining of human kidney using 16623-1-AP (same clone as 16623-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16623-1-AP (ACADS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16623-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ACADS Polyclonal antibody (16623-1-AP)

    IHC staining of human kidney using 16623-1-AP (same clone as 16623-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 16623-1-AP (ACADS antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16623-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ACADS Polyclonal antibody (16623-1-AP)

    IHC staining of human kidney using 16623-1-AP (same clone as 16623-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 16623-1-AP (ACADS antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16623-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ACADS Polyclonal antibody (16623-1-AP)

    IHC staining of human kidney using 16623-1-AP (same clone as 16623-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16623-1-AP (ACADS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16623-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using ACADS Polyclonal antibody (16623-1-AP)

    IHC staining of human heart using 16623-1-AP (same clone as 16623-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 16623-1-AP (ACADS antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16623-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using ACADS Polyclonal antibody (16623-1-AP)

    IHC staining of human heart using 16623-1-AP (same clone as 16623-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 16623-1-AP (ACADS antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16623-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ACADS Polyclonal antibody, PBS Only

ACADS Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 16623-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16623-1-PBS targets ACADS in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity human, mouse, rat, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9922

Product name: Recombinant human ACADS protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-412 aa of BC025963

Sequence: MAAALLARASGPARRALCPRAWRQLHTIYQSVELPETHQMLLQTCRDFAEKELFPIAAQVDKEHLFPAAQVKKMGGLGLLAMDVPEELGGAGLDYLAYAIAMEEISRGCASTGVIMSVNNSLYLGPILKFGSKEQKQAWVTPFTSGDKIGCFALSEPGNGSDAGAASTTARAEGDSWVLNGTKAWITNAWEASAAVVFASTDRALQNKSISAFLVPMPTPGLTLGKKEDKLGIRGSSTANLIFEDCRIPKDSILGEPGMGFKIAMQTLDMGRIGIASQALGIAQTALDCAVNYAENRMAFGAPLTKLQVIQFKLADMALALESARLLTWRAAMLKDNKKPFIKEAAMAKLAASEAATAISHQAIQILGGMGYVTEMPAERHYRDARITEIYEGTSEIQRLVIAGHLLRSYRS

相同性解析による交差性が予測される生物種
Full Name acyl-Coenzyme A dehydrogenase, C-2 to C-3 short chain
Calculated molecular weight 412 aa, 44 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC025963
Gene Symbol ACADS
Gene ID (NCBI) 35
RRIDAB_10666165
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP16219
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ACADS is also named as SCAD(Short-chain specific acyl-CoA dehydrogenase, mitochondrial) and belongs to the acyl-CoA dehydrogenase family. It is a tetrameric mitochondrial flavoprotein. It is synthesized as a 44 kDa precursor, transported into mitochondria, and proteolytically processed to its 41 kDa mature form. Acyl-CoA de- hydrogenases (ACADs) are a family of flavoproteins that are involved in theβ-oxidation of the fatty acyl-CoA derivatives.

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