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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACADM Monoclonal antibody (67742-1-Ig)

    WB analysis using 67742-1-Ig (same clone as 67742-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67742-1-Ig (ACADM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67742-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using ACADM Monoclonal antibody (67742-1-Ig)

    WB analysis of rat liver using 67742-1-Ig (same clone as 67742-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 67742-1-Ig (ACADM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67742-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using ACADM Monoclonal antibody (67742-1-Ig)

    WB analysis of HSC-T6 using 67742-1-Ig (same clone as 67742-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67742-1-Ig (ACADM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67742-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using ACADM Monoclonal antibody (67742-1-Ig)

    IHC staining of human liver using 67742-1-Ig (same clone as 67742-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67742-1-Ig (ACADM antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67742-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using ACADM Monoclonal antibody (67742-1-Ig)

    IHC staining of human liver using 67742-1-Ig (same clone as 67742-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67742-1-Ig (ACADM antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67742-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using ACADM Monoclonal antibody (67742-1-Ig)

    IHC staining of human heart using 67742-1-Ig (same clone as 67742-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67742-1-Ig (ACADM antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67742-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using ACADM Monoclonal antibody (67742-1-Ig)

    IHC staining of human heart using 67742-1-Ig (same clone as 67742-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67742-1-Ig (ACADM antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67742-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using ACADM Monoclonal antibody (67742-1-Ig)

    IF Staining of human liver cancer using 67742-1-Ig (same clone as 67742-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using ACADM antibody (67742-1-Ig, Clone: 1A11G6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67742-1-PBS in a different storage buffer formulation.

ACADM Monoclonal antibody, PBS Only

ACADM Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 67742-1-PBS
Clone No.1A11G6

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

1A11G6, ACAD, ACAD 1, ACAD1, EC:1.3.8.7

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67742-1-PBS targets ACADM in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, rat, pig samples.

Tested Reactivity human, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag30620

Product name: Recombinant human ACADM protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 205-352 aa of BC005377

Sequence: ARSDPDPKAPANKAFTGFIVEADTPGIQIGRKELNMGQRCSDTRGIVFEDVKVPKENVLIGDGAGFKVAMGAFDKTRPVVAAGAVGLAQRALDEATKYALERKTFGKLLVEHQAISFMLAEMAMKVELARMSYQRAAWEVDSGRRNTY

相同性解析による交差性が予測される生物種
Full Name acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain
Calculated molecular weight 421 aa, 47 kDa
Observed molecular weight42 kDa
GenBank accession numberBC005377
Gene Symbol ACADM
Gene ID (NCBI) 34
RRIDAB_2918511
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP11310
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ACADM, also named as MCAD, belongs to the acyl-CoA dehydrogenase family. This enzyme is specific for acyl chain lengths of 4 to 16. It catalyzes the reaction: Acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor. Defects in ACADM are the cause of medium-chain acyl-CoA dehydrogenase deficiency (MCAD deficiency). This protein can exsit as a dimer(PMID:8962055). This antibody is specific to ACADM.

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