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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACADM Polyclonal antibody (55210-1-AP)

    WB analysis using 55210-1-AP (same clone as 55210-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 55210-1-AP (ACADM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55210-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ACADM Polyclonal antibody (55210-1-AP)

    WB analysis using 55210-1-AP (same clone as 55210-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 55210-1-AP (ACADM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55210-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat heart tissue using ACADM Polyclonal antibody (55210-1-AP)

    WB analysis of rat heart using 55210-1-AP (same clone as 55210-1-PBS)

    rat heart tissue were subjected to SDS PAGE followed by western blot with 55210-1-AP (ACADM Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55210-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using ACADM Polyclonal antibody (55210-1-AP)

    WB analysis of K-562 using 55210-1-AP (same clone as 55210-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 55210-1-AP (ACADM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55210-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using ACADM Polyclonal antibody (55210-1-AP)

    WB analysis of HepG2 using 55210-1-AP (same clone as 55210-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 55210-1-AP (ACADM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55210-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using ACADM Polyclonal antibody (55210-1-AP)

    IHC staining of human liver using 55210-1-AP (same clone as 55210-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55210-1-AP (ACADM antibody) at dilution of 1:150 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55210-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using ACADM Polyclonal antibody (55210-1-AP)

    IHC staining of human liver using 55210-1-AP (same clone as 55210-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55210-1-AP (ACADM antibody) at dilution of 1:150 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55210-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using ACADM Polyclonal antibody (55210-1-AP)

    IHC staining of human heart using 55210-1-AP (same clone as 55210-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 55210-1-AP (ACADM antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55210-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using ACADM Polyclonal antibody (55210-1-AP)

    IHC staining of human heart using 55210-1-AP (same clone as 55210-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 55210-1-AP (ACADM antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55210-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ACADM Polyclonal antibody, PBS Only

ACADM Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 55210-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

ACAD, ACAD 1, ACAD1, EC:1.3.8.7, MCAD

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

55210-1-PBS targets ACADM in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain
Calculated molecular weight 47 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberNM_000016
Gene Symbol ACADM
Gene ID (NCBI) 34
RRIDAB_10837361
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP11310
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ACADM, also named as MCAD, belongs to the acyl-CoA dehydrogenase family. This enzyme is specific for acyl chain lengths of 4 to 16. It catalyzes the reaction: Acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor. Defects in ACADM are the cause of medium-chain acyl-CoA dehydrogenase deficiency (MCAD deficiency). This protein can exsit as a dimer(PMID:8962055). This antibody is specific to ACADM.

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