Validation Data Gallery

Filter: AllWBIHC

WB analysis using 11111-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11111-1-AP (ACAA2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11111-1-AP
WB result of ACAA2 antibody (11111-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACAA2 transfected HEK-293 cells.
IHC staining of human lung cancer using 11111-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11111-1-AP (ACAA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 11111-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11111-1-AP (ACAA2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 11111-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 11111-1-AP (ACAA2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 11111-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 11111-1-AP (ACAA2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of mouse liver using 11111-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11111-1-AP (ACAA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

ACAA2 Polyclonal antibody

ACAA2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 11111-1-AP

Publications(12)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IP, ELISA

Synonyms

Beta ketothiolase, Acyl-CoA hydrolase, mitochondrial, Acetyl-CoA acyltransferase, Acetyl-CoA acetyltransferase, Acetyl CoA acyltransferase

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (12)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, HepG2 cells, NIH/3T3 cells
Positive IHC detected in	human lung cancer tissue, human colon tissue, human liver tissue, mouse liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:6000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 12 publications below
IHC	See 2 publications below
IP	See 2 publications below

Product Information

11111-1-AP targets ACAA2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, sheep
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1609 Product name: Recombinant human ACAA2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 98-397 aa of BC001918 Sequence: SIVNGCQEICVKEAEVVLCGGTESMSQAPYCVRNVRFGTKLGSDIKLEDSLWVSLTDQHVQLPMAMTAENLAVKHKISREECDKYALQSQQRWKAANDAGYFNDEMAPIEVKTKKGKQTMQVDEHARPQTTLEQLQKLPPVFKKDGTVTAGNASGVADGAGAVIIASEDAVKKHNFTPLARIVGYFVSGCDPSIMGIGPVPAISGALKKAGLSLKDMDLVEVNEAFAPQYLAVERSLDLDISKTNVNGGAIALGHPLGGSGSRITAHLVHELRRRGGKYAVGSACIGGGQGIAVIIQSTA 相同性解析による交差性が予測される生物種
Full Name	acetyl-Coenzyme A acyltransferase 2
Calculated molecular weight	42 kDa
Observed molecular weight	42 kDa
GenBank accession number	BC001918
Gene Symbol	ACAA2
Gene ID (NCBI)	10449
RRID	AB_2219389
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P42765
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for ACAA2 antibody 11111-1-AP	Download protocol
WB protocol for ACAA2 antibody 11111-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Oncotarget Genomic binding and regulation of gene expression by the thyroid carcinoma-associated PAX8-PPARG fusion protein. Authors - Yanxiao Zhang View Article
rat	WB	EBioMedicine PPARγ maintains the metabolic heterogeneity and homeostasis of renal tubules. Authors - Zhongshi Lyu View Article
rat	WB,IHC	PLoS One Pressure load: the main factor for altered gene expression in right ventricular hypertrophy in chronic hypoxic rats. Authors - Baandrup Jonas D JD View Article
sheep	WB	Front Genet Identification of miRNA in Sheep Intramuscular Fat and the Role of miR-193a-5p in Proliferation and Differentiation of 3T3-L1. Authors - Fuhui Han View Article
rat	WB	Gerontology Dietary Restriction Increases Protein Acetylation in the Livers of Aged Rats. Authors - Nakamura Akihiro A View Article
human	WB	J Biol Chem CLPX regulates mitochondrial fatty acid β-oxidation in liver cells Authors - Ko Suzuki View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 11111-1-AP

WB analysis of HEK-293 using 11111-1-AP

IHC staining of human lung cancer using 11111-1-AP

IHC staining of human colon using 11111-1-AP

IHC staining of human liver using 11111-1-AP

IHC staining of human liver using 11111-1-AP

IHC staining of mouse liver using 11111-1-AP

ACAA2 Polyclonal antibody

ACAA2 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Genomic binding and regulation of gene expression by the thyroid carcinoma-associated PAX8-PPARG fusion protein.

PPARγ maintains the metabolic heterogeneity and homeostasis of renal tubules.

Pressure load: the main factor for altered gene expression in right ventricular hypertrophy in chronic hypoxic rats.

Identification of miRNA in Sheep Intramuscular Fat and the Role of miR-193a-5p in Proliferation and Differentiation of 3T3-L1.

Dietary Restriction Increases Protein Acetylation in the Livers of Aged Rats.

CLPX regulates mitochondrial fatty acid β-oxidation in liver cells