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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACAA1 Recombinant monoclonal antibody (87969-1-RR)

    WB analysis using 87969-1-RR (same clone as 87969-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87969-1-RR (ACAA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87969-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ACAA1 Recombinant monoclonal antibody (87969-1-RR)

    WB analysis using 87969-1-RR (same clone as 87969-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87969-1-RR (ACAA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87969-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ACAA1 Recombinant monoclonal antibody (87969-1-RR)

    WB analysis using 87969-1-RR (same clone as 87969-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87969-1-RR (ACAA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87969-1-PBS in a different storage buffer formulation.

ACAA1 Recombinant monoclonal antibody, PBS Only

ACAA1 Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 87969-1-PBS
Clone No.260194F9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

3 ketoacyl CoA thiolase, peroxisomal, 3-ketoacyl-CoA thiolase, peroxisomal, ACAA, Acetyl CoA acyltransferase, Acetyl-CoA acyltransferase

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

87969-1-PBS targets ACAA1 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag2972

Product name: Recombinant human ACAA1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 117-424 aa of BC011977

Sequence: STVNRQCSSGLQAVASIAGGIRNGSYDIGMACGVESMSLADRGNPGNITSRLMEKEKARDCLIPMGITSENVAERFGISREKQDTFALASQQKAARAQSKGCFQAEIVPVTTTVHDDKGTKRSITVTQDEGIRPSTTMEGLAKLKPAFKKDGSTTAGNSSQVSDGAAAILLARRSKAEELGLPILGVLRSYAVVGVPPDIMGIGPAYAIPVALQKAGLTVSDVDIFEINEAFASQAAYCVEKLRLPPEKVNPLGGAVALGHPLGCTGARQVITLLNELKRRGKRAYGVVSMCIGTGMGAAAVFEYPGN

相同性解析による交差性が予測される生物種
Full Name acetyl-Coenzyme A acyltransferase 1
Calculated molecular weight 424 aa, 44 kDa
Observed molecular weight41 kDa
GenBank accession numberBC011977
Gene Symbol ACAA1
Gene ID (NCBI) 30
RRIDAB_3745839
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP09110
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ACAA1 (acetyl-CoA acyltransferase 1) is an enzyme involved in lipid β-oxidation and provides substrates to the tricarboxylic acid (TCA) cycle, a critical step in cellular metabolism (PMID:33194642). ACAA1 is also a biomarker in type 2 diabetes (T2D), predicting the pre-diabetic metabolic signature in mouse models (PMID:25784953). Moreover, ACAA1 is highly expressed in TNBC, serving as a potential therapeutic target in ACAA1-high tumors and a predictive biomarker of resistance to CDK4/6 inhibitors for RB1-proficient patients (PMID:37129951).

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