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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using ABT1 Polyclonal antibody (14148-1-AP)

    WB analysis of mouse liver using 14148-1-AP (same clone as 14148-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 14148-1-AP (ABT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14148-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ABT1 Polyclonal antibody (14148-1-AP)

    WB analysis of HeLa using 14148-1-AP (same clone as 14148-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 14148-1-AP (ABT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14148-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using ABT1 Polyclonal antibody (14148-1-AP)

    IHC staining of human brain using 14148-1-AP (same clone as 14148-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 14148-1-AP (ABT1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14148-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using ABT1 Polyclonal antibody (14148-1-AP)

    IHC staining of human brain using 14148-1-AP (same clone as 14148-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 14148-1-AP (ABT1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14148-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ABT1 Polyclonal antibody (14148-1-AP)

    IF Staining of HeLa using 14148-1-AP (same clone as 14148-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ABT1 antibody (14148-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 14148-1-PBS in a different storage buffer formulation.

ABT1 Polyclonal antibody, PBS Only

ABT1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 14148-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

hABT1, Esf2, Activator of basal transcription 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14148-1-PBS targets ABT1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5317

Product name: Recombinant human ABT1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-272 aa of BC066313

Sequence: MEAEESEKAATEQEPLEGTEQTLDAEEEQEESEEAACGSKKRVVPGIVYLGHIPPRFRPLHVRNLLSAYGEVGRVFFQAEDRFVRRKKKAAAAAGGKKRSYTKDYTEGWVEFRDKRIAKRVAASLHNTPMGARRRSPFRYDLWNLKYLHRFTWSHLSEHLAFERQVRRQRLRAEVAQAKRETDFYLQSVERGQRFLAADGDPARPDGSWTFAQRPTEQELRARKAARPGGRERARLATAQDKARSNKGLLARIFGAPPPSESMEGPSLVRDS

相同性解析による交差性が予測される生物種
Full Name activator of basal transcription 1
Calculated molecular weight 31 kDa
Observed molecular weight 31-32 kDa
GenBank accession numberBC066313
Gene Symbol ABT1
Gene ID (NCBI) 29777
RRIDAB_10640440
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9ULW3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.