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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ABR Polyclonal antibody (29350-1-AP)

    WB analysis using 29350-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29350-1-AP (ABR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-251 cells using ABR Polyclonal antibody (29350-1-AP)

    WB analysis of U-251 using 29350-1-AP

    WB result of ABR antibody (29350-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ABR transfected U-251 cells.

  • Immunohistochemistry (IHC) staining of rat brain tissue using ABR Polyclonal antibody (29350-1-AP)

    IHC staining of rat brain using 29350-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 29350-1-AP (ABR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using ABR Polyclonal antibody (29350-1-AP)

    IHC staining of rat brain using 29350-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 29350-1-AP (ABR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO Validated

ABR Polyclonal antibody

ABR Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29350-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

active BCR related gene, MDB

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSH-SY5Y cells, U-251 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29350-1-AP targets ABR in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag29522

Product name: Recombinant human ABR protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-87 aa of NM_021962

Sequence: MEPLSHRGLPRLSWIDTLYSNFSYGTDEYDGEGNEEQKGPPEGSETMPYIDESPTMSPQLSARSQGGGDGVSPTPPEGLAPGVEAGK

相同性解析による交差性が予測される生物種
Full Name active BCR-related gene
Calculated molecular weight 98 kDa
Observed molecular weight97-100 kDa
GenBank accession numberNM_021962
Gene Symbol ABR
Gene ID (NCBI) 29
RRIDAB_3086122
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12979
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ABR (Active breakpoint cluster region-related protein) is the only protein known in humans and mice to share high homology with BCR (68% amino acid identity). BCR(Breakpoint cluster region) gene was originally identified due to its involvement in a specific chromosomal translocation that causes the development of chronic myeloid leukemia and a subset of acute lymphoblastic leukemia. The C-terminus is a GTPase-activating protein domain which stimulates GTP hydrolysis by RAC1, RAC2 and CDC42. (PMID: 17116687, PMID: 37507586)

Protocols

Product Specific Protocols
WB protocol for ABR antibody 29350-1-APDownload protocol
IHC protocol for ABR antibody 29350-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols