Validation Data Gallery

Filter: AllWBIHC

WB analysis using 14890-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14890-1-AP (ABI2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 14890-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 14890-1-AP (ABI2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 14890-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 14890-1-AP (ABI2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 14890-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 14890-1-AP (ABI2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 14890-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 14890-1-AP (ABI2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse uterus using 14890-1-AP
mouse uterus tissue were subjected to SDS PAGE followed by western blot with 14890-1-AP (ABI2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human brain using 14890-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14890-1-AP (ABI2 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human brain using 14890-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14890-1-AP (ABI2 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human placenta using 14890-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14890-1-AP (ABI2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 14890-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14890-1-AP (ABI2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 14890-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14890-1-AP (ABI2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat pancreas using 14890-1-AP
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 14890-1-AP (ABI2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 14890-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14890-1-AP (ABI2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human testis using 14890-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14890-1-AP (ABI2 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 14890-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14890-1-AP (ABI2 Antibody) at dilution of 1:50 (under 40x lens).

ABI2 Polyclonal antibody

ABI2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 14890-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, CoIP, ELISA

Synonyms

Abelson interactor 2, Abi-2, Abl binding protein 3, Abl-binding protein 3, AblBP3

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, mouse heart tissue, mouse uterus tissue, mouse testis tissue, mouse brain tissue, HeLa cells, K-562 cells, SH-SY5Y cells
Positive IHC detected in	human brain tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:6000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
CoIP	See 1 publications below

Product Information

14890-1-AP targets ABI2 in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6679 Product name: Recombinant human ABI2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-275 aa of BC001439 Sequence: MAELQMLLEEEIPGGRRALFDSYTNLERVADYCENNYIQSADKQRALEETKAYTTQSLASVAYLINTLANNVLQMLDIQASQLRRMESSINHISQTVDIHKEKVARREIGILTTNKNTSRTHKIIAPANLERPVRYIRKPIDYTILDDIGHGVKVSTQNMKMGGLPRTTPPTQKPPSPPMSGKGTLGRHSPYRTLEPVRPPVVPNDYVPSPTRNMAPSQQSPVRTASVNQRNRTYSSSGSSGGSHPSSRSSSRENSGSGSVGVPIAVPTPSPPSV 相同性解析による交差性が予測される生物種
Full Name	abl interactor 2
Calculated molecular weight	56 kDa
Observed molecular weight	52-56 kDa
GenBank accession number	BC001439
Gene Symbol	ABI2
Gene ID (NCBI)	10152
RRID	AB_2288997
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NYB9
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for ABI2 antibody 14890-1-AP	Download protocol
WB protocol for ABI2 antibody 14890-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,CoIP	Nat Commun GRAF1 integrates PINK1-Parkin signaling and actin dynamics to mediate cardiac mitochondrial homeostasis Authors - Qiang Zhu View Article
human	WB	Nat Cell Biol Actin cytoskeleton vulnerability to disulfide stress mediates disulfidptosis Authors - Xiaoguang Liu View Article
mouse,human	WB	Nat Commun hnRNP A1 dysfunction alters RNA splicing and drives neurodegeneration in multiple sclerosis (MS) Authors - Hannah E Salapa View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 14890-1-AP

WB analysis of mouse brain using 14890-1-AP

WB analysis of mouse testis using 14890-1-AP

WB analysis of HEK-293 using 14890-1-AP

WB analysis of mouse heart using 14890-1-AP

WB analysis of mouse uterus using 14890-1-AP

IHC staining of human brain using 14890-1-AP

IHC staining of human brain using 14890-1-AP

IHC staining of human placenta using 14890-1-AP

IHC staining of mouse testis using 14890-1-AP

IHC staining of rat brain using 14890-1-AP

IHC staining of rat pancreas using 14890-1-AP

IHC staining of rat brain using 14890-1-AP

IHC staining of human testis using 14890-1-AP

IHC staining of human testis using 14890-1-AP

ABI2 Polyclonal antibody

ABI2 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

GRAF1 integrates PINK1-Parkin signaling and actin dynamics to mediate cardiac mitochondrial homeostasis

Actin cytoskeleton vulnerability to disulfide stress mediates disulfidptosis

hnRNP A1 dysfunction alters RNA splicing and drives neurodegeneration in multiple sclerosis (MS)

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