Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, HepG2 cells, LNCap cells, SiHa cells, mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32036-1-AP targets ABHD4 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag36373 Product name: Recombinant human ABHD4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 250-342 aa of BC024779 Sequence: PSGETAFKAMMESFGWARRPMLERIHLIRKDVPITMIYGSDTWIDTSTGKKVKMQRPDSYVRDMEIKGASHHVYADQPHIFNAVVEEICDSVD 相同性解析による交差性が予測される生物種 |
| Full Name | abhydrolase domain containing 4 |
| Calculated molecular weight | 342 aa, 39 kDa |
| Observed molecular weight | 39 kDa |
| GenBank accession number | BC024779 |
| Gene Symbol | ABHD4 |
| Gene ID (NCBI) | 63874 |
| RRID | AB_3670176 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q8TB40 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The alpha/beta hydrolase structural domain-containing protein 4 (ABHD4) gene is a major regulator of mammalian phospholipid metabolism with hydrolase and lysophospholipase activities. ABHD4 is involved in anoikis resistance and endogenous cannabinoid biosynthesis, and ABHD4-mediated developmental anoikis specifically protects embryonic brains from the consequences of sporadic delamination errors and teratogenic damage.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ABHD4 antibody 32036-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |