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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ABHD17A Polyclonal antibody (32439-1-AP)

    WB analysis using 32439-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32439-1-AP (ABHD17A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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ABHD17A Polyclonal antibody

ABHD17A Polyclonal Antibody for WB, ELISA
Cat No. 32439-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32439-1-AP targets ABHD17A in applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ABHD17A fusion protein Ag36531 相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 108, member A1
Calculated molecular weight 34 kDa
Observed molecular weight34 kDa
GenBank accession numberBC000158
Gene Symbol ABHD17A
Gene ID (NCBI) 81926
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ96GS6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Alpha/beta hydrolase domain-containing protein 17A (ABHD17A), also known as FAM108A, is a membrane-localized thioesterase, which regulates H- and N-RAS39, also catalyzes the depalmitoylation of CNAβ1 causing it to redistribute from the PM to the cytosol and the Golgi (PMID: 34663815). ABHD17a is a major acyl protein thioesterase that site-specifically deacylates the STREX domain of the BK channel to control channel activity (PMID: 32913120). Western blot analysis detected ABHD17A at an apparent molecular mass of 34 kDa (PMID: 27307232), and the 70 kDa-band could be the dimer.

Protocols

Product Specific Protocols
WB protocol for ABHD17A antibody 32439-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols