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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ABHD12B Polyclonal antibody

ABHD12B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20623-1-AP

Synonyms

ABHD12B, BEM46L3, c14_5314, C14orf29

Validation Data Gallery

Applications:
  • WB analysis of mouse skin using 20623-1-AP

    WB analysis of mouse skin using 20623-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 20623-1-AP (ABHD12B antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse kidney using 20623-1-AP

    WB analysis of mouse kidney using 20623-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20623-1-AP (ABHD12B antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin using 20623-1-AP

    IHC staining of human skin using 20623-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 20623-1-AP (ABHD12B antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skin using 20623-1-AP

    IHC staining of human skin using 20623-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 20623-1-AP (ABHD12B antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human kidney using 20623-1-AP

    IHC staining of human kidney using 20623-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 20623-1-AP (ABHD12B antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 20623-1-AP

    IHC staining of human kidney using 20623-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 20623-1-AP (ABHD12B antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse skin tissue, mouse kidney tissue
Positive IHC detected inhuman skin tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20623-1-AP is ABHD12B Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ABHD12B fusion protein Ag14426 相同性解析による交差性が予測される生物種
Full Name abhydrolase domain containing 12B
Calculated molecular weight 362 aa, 41 kDa
Observed molecular weight 41 kDa, 29 kDa
GenBank accession numberBC034603
Gene symbol ABHD12B
Gene ID (NCBI) 145447
RRIDAB_10693623
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ABHD12B antibody 20623-1-APDownload protocol
IHC protocol for ABHD12B antibody 20623-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols