Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse cerebellum tissue, rat cerebellum tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22660-1-AP targets ABHD12 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18522 Product name: Recombinant human ABHD12 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 88-404 aa of BC014049 Sequence: FLIKLCPGIQAKLIFLNFVRVPYFIDLKKPQDQGLNHTCNYYLQPEEDVTIGVWHTVPAVWWKNAQGKDQMWYEDALASSHPIILYLHGNAGTRGGDHRVELYKVLSSLGYHVVTFDYRGWGDSVGTPSERGMTYDALHVFDWIKARSGDNPVYIWGHSLGTGVATNLVRRLCERETPPDALILESPFTNIREEAKSHPFSVIYRYFPGFDWFFLDPITSSGIKFANDENVKHISCPLLILHAEDDPVVPFQLGRKLYSIAAPARSFRDFKVQFVPFHSDLGYRHKYIYKSPELPRILRPQQGPGSSPDPSMWSELV 相同性解析による交差性が予測される生物種 |
| Full Name | abhydrolase domain containing 12 |
| Calculated molecular weight | 404 aa, 46 kDa |
| Observed molecular weight | 42-45 kDa |
| GenBank accession number | BC014049 |
| Gene Symbol | ABHD12 |
| Gene ID (NCBI) | 26090 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N2K0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ABHD12 is a recently identified post-genomic protein with poorly defined physiological function. Its potential role as a brain 2-AG hydrolase was revealed using ABPP with mouse brain proteome and it was estimated that at the bulk brain level. ABHD12 is a major brain lyso-PS lipase, implicating lyso-PSs in the neurological disease PHARC, which is caused by null mutations in the ABHD12 gene (PMID: 21418147, 25580854).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ABHD12 antibody 22660-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |