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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ABHD11 Polyclonal antibody (25355-1-AP)

    WB analysis using 25355-1-AP (same clone as 25355-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25355-1-AP (ABHD11 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25355-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using ABHD11 Polyclonal antibody (25355-1-AP)

    IF Staining of PC-3 using 25355-1-AP (same clone as 25355-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using ABHD11 antibody (25355-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 25355-1-PBS in a different storage buffer formulation.

ABHD11 Polyclonal antibody, PBS Only

ABHD11 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 25355-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

sn-1-specific diacylglycerol lipase ABHD11, PP1226, EC:3.1.1.116, Alpha/beta hydrolase domain-containing protein 11, Abhydrolase domain-containing protein 11

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25355-1-PBS targets ABHD11 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag18318

Product name: Recombinant human ABHD11 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-315 aa of BC067750

Sequence: MRAGQQLASMLRWTRAWRLPREGLGPHGPSFARVPVAPSSSSGGRGGAEPRPLPLSYRLLDGEAALPAVVFLHGLFGSKTNFNSIAKILAQQTGRRVLTVDARNHGDSPHSPDMSYEIMSQDLQDLLPQLGLVPCVVVGHSMGGKTAMLLALQRPELVERLIAVDISPVESTGVSHFATYVAAMRAINIADELPRSRARKLADEQLSSVIQDMAVRQHLLTNLVEVDGRFVWRVNLDALTQHLDKILAFPQRQESYLGPTLFLLGGNSQFVHPSHHPEIMRLFPRAQMQTVPNAGHWIHADRPQDFIAAIRGFLV

相同性解析による交差性が予測される生物種
Full Name abhydrolase domain containing 11
Calculated molecular weight 315 aa, 35 kDa
Observed molecular weight30 kDa
GenBank accession numberBC067750
Gene Symbol ABHD11
Gene ID (NCBI) 83451
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NFV4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Abhydrolase domain-containing protein 11 (ABHD11) is a serine hydrolase enzyme, localized in mitochondria, that can synthesize the endocannabinoid 2-arachidonoyl glycerol (2AG) in vitro.

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