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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ABHD10 Polyclonal antibody (22558-1-AP)

    WB analysis of HeLa using 22558-1-AP

    WB result of ABHD10 antibody (22558-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ABHD10 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using ABHD10 Polyclonal antibody (22558-1-AP)

    WB analysis using 22558-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 22558-1-AP (ABHD10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  • KD/KO Validated

ABHD10 Polyclonal antibody

ABHD10 Polyclonal Antibody for WB, ELISA
Cat No. 22558-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inY79 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22558-1-AP targets ABHD10 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ABHD10 fusion protein Ag18413 相同性解析による交差性が予測される生物種
Full Name abhydrolase domain containing 10
Calculated molecular weight 306 aa, 34 kDa
Observed molecular weight28 kDa
GenBank accession numberBC014516
Gene Symbol ABHD10
Gene ID (NCBI) 55347
RRIDAB_3669401
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NUJ1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ABHD10 is a mitochondrial protein that is a member of the APT family. It has 3 isoforms and regulates the S-palmitoylation status of the nucleophilic active site residue of peroxiredoxin-5 (PRDX5) (PMID: 31740833).

Protocols

Product Specific Protocols
WB protocol for ABHD10 antibody 22558-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols