Validation Data Gallery
Tested Applications
| Positive WB detected in | Y79 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22558-1-AP targets ABHD10 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18413 Product name: Recombinant human ABHD10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-306 aa of BC014516 Sequence: MAVARLAAVAAWVPCRSWGWAAVPFGPHRGLSVLLARIPQRAPRWLPACRQKTSLSFLNRPDLPNLAYKKLKGKSPGIIFIPGYLSYMNGTKALAIEEFCKSLGHACIRFDYSGVGSSDGNSEESTLGKWRKDVLSIIDDLADGPQILVGSSLGGWLMLHAAIARPEKVVALIGVATAADTLVTKFNQLPVELKKEVEMKGVWSMPSKYSEEGVYNVQYSFIKEAEHHCLLHSPIPVNCPIRLLHGMKDDIVPWHTSMQVADRVLSTDVDVILRKHSDHRMREKADIQLLVYTIDDLIDKLSTIVN 相同性解析による交差性が予測される生物種 |
| Full Name | abhydrolase domain containing 10 |
| Calculated molecular weight | 306 aa, 34 kDa |
| Observed molecular weight | 28 kDa |
| GenBank accession number | BC014516 |
| Gene Symbol | ABHD10 |
| Gene ID (NCBI) | 55347 |
| RRID | AB_3669401 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NUJ1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ABHD10 is a mitochondrial protein that is a member of the APT family. It has 3 isoforms and regulates the S-palmitoylation status of the nucleophilic active site residue of peroxiredoxin-5 (PRDX5) (PMID: 31740833).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ABHD10 antibody 22558-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |