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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ABCG4 Polyclonal antibody

ABCG4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14269-1-AP

Synonyms

ABCG4, WHITE2

Validation Data Gallery

Applications:
  • WB analysis of U-937 using 14269-1-AP

    WB analysis of U-937 using 14269-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 14269-1-AP (ABCG4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 14269-1-AP

    WB analysis of mouse brain using 14269-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14269-1-AP (ABCG4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of U-937 using 14269-1-AP

    WB analysis of U-937 using 14269-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 14269-1-AP (ABCG4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 14269-1-AP

    IHC staining of human liver cancer using 14269-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 14269-1-AP (ABCG4 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver cancer using 14269-1-AP

    IHC staining of human liver cancer using 14269-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 14269-1-AP (ABCG4 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inU-937 cells, mouse brain tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14269-1-AP targets ABCG4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ABCG4 fusion protein Ag5587 相同性解析による交差性が予測される生物種
Full Name ATP-binding cassette, sub-family G (WHITE), member 4
Calculated molecular weight 72 kDa
Observed molecular weight 60 kDa, 90-100 kDa
GenBank accession numberBC041091
Gene symbol ABCG4
Gene ID (NCBI) 64137
RRIDAB_2220436
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ABCG4 antibody 14269-1-APDownload protocol
IHC protocol for ABCG4 antibody 14269-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB, IHC

PLoS One

High ABCG4 Expression Is Associated with Poor Prognosis in Non-Small-Cell Lung Cancer Patients Treated with Cisplatin-Based Chemotherapy.

Authors - Guang Yang
View Article