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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ABCF1 Polyclonal antibody (13950-1-AP)

    WB analysis using 13950-1-AP (same clone as 13950-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13950-1-AP (ABCF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13950-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using ABCF1 Polyclonal antibody (13950-1-AP)

    WB analysis of K-562 using 13950-1-AP (same clone as 13950-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 13950-1-AP (ABCF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13950-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of K-562 cells using ABCF1 Polyclonal antibody (13950-1-AP)

    IP experiment of K-562 using 13950-1-AP (same clone as 13950-1-PBS)

    IP result of anti-ABCF1 (IP:13950-1-AP, 4ug; Detection:13950-1-AP 1:6000) with K-562 cells lysate 1360 ug. This data was developed using the same antibody clone with 13950-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using ABCF1 Polyclonal antibody (13950-1-AP)

    IHC staining of human stomach cancer using 13950-1-AP (same clone as 13950-1-PBS)

    Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 13950-1-AP (ABCF1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13950-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using ABCF1 Polyclonal antibody (13950-1-AP)

    IHC staining of human stomach cancer using 13950-1-AP (same clone as 13950-1-PBS)

    Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 13950-1-AP (ABCF1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13950-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ABCF1 Polyclonal antibody (13950-1-AP)

    IF Staining of HeLa using 13950-1-AP (same clone as 13950-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ABCF1 antibody (13950-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13950-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ABCF1 Polyclonal antibody (13950-1-AP)

    IF Staining of HeLa using 13950-1-AP (same clone as 13950-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13950-1-AP (ABCF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13950-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ABCF1 Polyclonal antibody, PBS Only

ABCF1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 13950-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

TNF-alpha-stimulated ABC protein, ATP-binding cassette sub-family F member 1, ATP-binding cassette 50, ATP binding cassette 50, ABCF 1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13950-1-PBS targets ABCF1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5061

Product name: Recombinant human ABCF1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 496-845 aa of BC034488

Sequence: GWRKTLLIVSHDQGFLDDVCTDIIHLDAQRLHYYRGNYMTFKKMYQQKQKELLKQYEKQEKKLKELKAGGKSTKQAEKQTKEALTRKQQKCRRKNQDEESQEAPELLKRPKEYTVRFTFPDPPPLSPPVLGLHGVTFGYQGQKPLFKNLDFGIDMDSRICIVGPNGVGKSTLLLLLTGKLTPTHGEMRKNHRLKIGFFNQQYAEQLRMEETPTEYLQRGFNLPYQDARKCLGRFGLESHAHTIQICKLSGGQKARVVFAELACREPDVLILDEPTNNLDIESIDALGEAINEYKGAVIVVSHDARLITETNCQLWVVEEQSVSQIDGDFEDYKREVLEALGEVMVSRPRE

相同性解析による交差性が予測される生物種
Full Name ATP-binding cassette, sub-family F (GCN20), member 1
Calculated molecular weight 92 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC034488
Gene Symbol ABCF1
Gene ID (NCBI) 23
RRIDAB_2242044
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NE71
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ABCF1, also known as ABC27, and ABC50, is a member of the superfamily of ATP-binding cassette (ABC) transporters and contains two ABC transporter domains. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). Unlike other members of the superfamily, ABCF1 lacks the transmembrane domains that are characteristic of most ABC transporters. ABCF1 may be regulated by tumor necrosis factor-alpha and play a role in the enhancement of protein synthesis and thus participate in the inflammatory processes(PMID: 9790762).

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