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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using ABCD3 Recombinant monoclonal antibody (87047-1-RR)

    WB analysis of mouse liver using 87047-1-RR (same clone as 87047-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 87047-1-RR (ABCD3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87047-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ABCD3 Recombinant monoclonal antibody (87047-1-RR)

    WB analysis using 87047-1-RR (same clone as 87047-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87047-1-RR (ABCD3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87047-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using ABCD3 Recombinant monoclonal antibody (87047-1-RR)

    WB analysis of HEK-293 using 87047-1-RR (same clone as 87047-1-PBS)

    WB result of ABCD3 antibody (87047-1-RR; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-ABCD3 transfected HEK-293 cells. This data was developed using the same antibody clone with 87047-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using ABCD3 Recombinant monoclonal antibody (87047-1-RR)

    IF Staining of PC-3 using 87047-1-RR (same clone as 87047-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using ABCD3 antibody (87047-1-RR, Clone: 252233H1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87047-1-PBS in a different storage buffer formulation.

ABCD3 Recombinant monoclonal antibody, PBS Only

ABCD3 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 87047-1-PBS
Clone No.252233H1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

ATP-binding cassette sub-family D member 3, EC:3.1.2.-, PMP70, PXMP1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

87047-1-PBS targets ABCD3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag24760

Product name: Recombinant human ABCD3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-124 aa of BC068509

Sequence: MAAFSKYLTARNSSLAGAAFLLLCLLHKRRRALGLHGKKSGKPPLQNNEKEGKKERAVVDKVFFSRLIQILKIMVPRTFCKETGYLVLIAVMLVSRTYCDVWMIQNGTLIESGIIGRSRKDFKR

相同性解析による交差性が予測される生物種
Full Name ATP-binding cassette, sub-family D (ALD), member 3
Observed molecular weight 70 kDa
GenBank accession numberBC068509
Gene Symbol ABCD3
Gene ID (NCBI) 5825
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP28288
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ABCD3 belongs to the superfamily of ATP-binding cassette (ABC) transporters and is a peroxisomal membrane transporter involved in the transport of branched-chain fatty acids and C27 bile acids into the peroxisome. ABCD3 plays a role in the regulation of LCFAs and energy metabolism, namely in the degradation and biosynthesis of fatty acids via beta-oxidation (PMID: 24333844). Mutations in ABCD3 are associated with congenital bile acid synthesis defect 5 (CBAS5)(PMID: 25168382).

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