• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ABCB9 Polyclonal antibody

ABCB9 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11066-1-AP

Synonyms

ABC transporter 9 protein, ABCB9, EST122234, hABCB9, KIAA1520, TAP like protein, TAPL

Validation Data Gallery

Applications:
  • WB analysis of BGC-823 using 11066-1-AP

    WB analysis of BGC-823 using 11066-1-AP

    BGC-823 cells were subjected to SDS PAGE followed by western blot with 11066-1-AP (ABCB9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 11066-1-AP

    WB analysis of K-562 using 11066-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 11066-1-AP (ABCB9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse thymus using 11066-1-AP

    WB analysis of mouse thymus using 11066-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11066-1-AP (ABCB9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of HL-60 using 11066-1-AP

    WB analysis of HL-60 using 11066-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 11066-1-AP (ABCB9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of human testis using 11066-1-AP

    WB analysis of human testis using 11066-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 11066-1-AP (ABCB9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse thymus using 11066-1-AP

    IP experiment of mouse thymus using 11066-1-AP

    IP result of anti-ABCB9 (IP:11066-1-AP, 4ug; Detection:11066-1-AP 1:500) with mouse thymus tissue lysate 4000 ug.

  • IHC staining of mouse testis using 11066-1-AP

    IHC staining of mouse testis using 11066-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11066-1-AP (ABCB9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ABCB9 antibody included
  • IHC staining of mouse testis using 11066-1-AP

    IHC staining of mouse testis using 11066-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11066-1-AP (ABCB9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ABCB9 antibody included
  • IF Staining of HepG2 using 11066-1-AP

    IF Staining of HepG2 using 11066-1-AP

    Immunofluorescent analysis of HepG2 cells, using ABCB9 antibody 11066-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inBGC-823 cells, HL-60 cells, human testis tissue, K-562 cells, mouse thymus tissue
Positive IP detected inmouse thymus tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ABCB9 Ready-To-Use IHC Kit. ABCB9 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

11066-1-AP targets ABCB9 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ABCB9 fusion protein Ag1542 相同性解析による交差性が予測される生物種
Full Name ATP-binding cassette, sub-family B (MDR/TAP), member 9
Calculated molecular weight 84 kDa
Observed molecular weight 72 kDa
GenBank accession numberBC017348
Gene symbol ABCB9
Gene ID (NCBI) 23457
RRIDAB_10898357
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ABCB9 antibody 11066-1-APDownload protocol
IHC protocol for ABCB9 antibody 11066-1-APDownload protocol
IF protocol for ABCB9 antibody 11066-1-APDownload protocol
IP protocol for ABCB9 antibody 11066-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols