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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AATF Polyclonal antibody (10282-1-AP)

    WB analysis using 10282-1-AP (same clone as 10282-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10282-1-AP (AATF antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10282-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using AATF Polyclonal antibody (10282-1-AP)

    WB analysis of HepG2 using 10282-1-AP (same clone as 10282-1-PBS)

    WB result of AATF antibody (10282-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AATF transfected HepG2 cells. This data was developed using the same antibody clone with 10282-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using AATF Polyclonal antibody (10282-1-AP)

    WB analysis of mouse brain using 10282-1-AP (same clone as 10282-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10282-1-AP (AATF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10282-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using AATF Polyclonal antibody (10282-1-AP)

    IHC staining of human ovary cancer using 10282-1-AP (same clone as 10282-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 10282-1-AP (AATF antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10282-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using AATF Polyclonal antibody (10282-1-AP)

    IF Staining of A431 using 10282-1-AP (same clone as 10282-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using AATF antibody (10282-1-AP) at dilution of 1:1500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10282-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

AATF Polyclonal antibody, PBS Only

AATF Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 10282-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Apoptosis-antagonizing transcription factor, CHE 1, CHE1, DED, HSPC277

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10282-1-PBS targets AATF in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0195

Product name: Recombinant human AATF protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 7-212 aa of BC000591

Sequence: LALQLEQLLNPRPSEADPEADPEEATAARVIDRFDEGEDGEGDFLVVGSIRKLASASLLDTDKRYCGKTTSRKAWNEDHWEQTLPGSSDEEISDEEGSGDEDSEGLGLEEYDEDDLGAAEEQECGDHRESKKSRSHSAKTPGFSVQSISDFEKFTKGMDDLGSSEEEEDEESGMEEGDDAEDSQGESEEDRAGDRNSEDDGVVMTF

相同性解析による交差性が予測される生物種
Full Name apoptosis antagonizing transcription factor
Calculated molecular weight 63 kDa
Observed molecular weight80-95 kDa
GenBank accession numberBC000591
Gene Symbol AATF
Gene ID (NCBI) 26574
RRIDAB_2219887
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NY61
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Apoptosis antagonizing transcription factor (AATF) is a nuclear phosphoprotein of 523 amino acids and contains an extremely acidic domain and a putative leucine zipper characteristic of transcription factors. AATF was identified on the basis of its interaction with MAP3K12/DLK, a protein kinase known to be involved in the induction of cell apoptosis. Overexpression of this gene interfered with MAP3K12 induced apoptosis. AATF also binds Rb and the core of pol II, and may be part of transcription regulatory complex.

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