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Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using AASDHPPT Polyclonal antibody (11244-1-AP)

    WB analysis of human brain using 11244-1-AP (same clone as 11244-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 11244-1-AP (AASDHPPT antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11244-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using AASDHPPT Polyclonal antibody (11244-1-AP)

    WB analysis of human brain using 11244-1-AP (same clone as 11244-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 11244-1-AP (AASDHPPT antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11244-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using AASDHPPT Polyclonal antibody (11244-1-AP)

    WB analysis of human brain using 11244-1-AP (same clone as 11244-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 11244-1-AP (AASDHPPT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11244-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AASDHPPT Polyclonal antibody (11244-1-AP)

    IF Staining of HeLa using 11244-1-AP (same clone as 11244-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AASDHPPT antibody (11244-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11244-1-PBS in a different storage buffer formulation.

AASDHPPT Polyclonal antibody, PBS Only

AASDHPPT Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 11244-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

CGI-80, CGI 80, Alpha-aminoadipic semialdehyde dehydrogenase-phosphopantetheinyl transferase, AASD-PPT, AASD PPT

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11244-1-PBS targets AASDHPPT in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1763

Product name: Recombinant human AASDHPPT protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-309 aa of BC016728

Sequence: MVFPAKRFCLVPSMEGVRWAFSCGTWLPSRAEWLLAVRSIQPEEKERIGQFVFARDAKAAMAGRLMIRKLVAEKLNIPWNHIRLQRTAKGKPVLAKDSSNPYPNFNFNISHQGDYAVLAAEPELQVGIDIMKTSFPGRGSIPEFFHIMKRKFTNKEWETIRSFKDEWTQLDMFYRNWALKESFIKAIGVGLGFELQRLEFDLSPLNLDIGQVYKETRLFLDGEEEKEWAFEESKIDEHHFVAVALRKPDGSRHQDVPSQDDSKPTQRQFTILNFNDLMSSAVPMTPEDPSFWDCFCFTEEIPIRNGTKS

相同性解析による交差性が予測される生物種
Full Name aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC016728
Gene Symbol AASDHPPT
Gene ID (NCBI) 60496
RRIDAB_2304862
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRN7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

AASDHPPT, also known as L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase, is required for mitochondrial respiration and oxidative metabolism via the mitochondrial fatty acid synthesis (mtFAS) pathway. AASDHPPT catalyzes the post-translational modification of target proteins by phosphopantetheine and transfer the 4'-phosphopantetheine moiety from coenzyme A to a serine residue of a broad range of acceptors, including the acyl carrier domain of FASN.

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