Validation Data Gallery
Tested Applications
| Positive WB detected in | PC-3 cells, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87679-2-RR targets AADACL1 in WB, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag5136 Product name: Recombinant human AADACL1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 83-435 aa of BC047588 Sequence: SHHLLALNFIIVSFGKKSAWSSAKVKVTDTDFDGVEVRVFEGPPKPEEPLKRSVVYIHGGGWALASAKIRYYDELCTAMAEELNAVIVSIEYRLVPKVYFPEQIHDVVRATKYFLKPEVLQKYMVDPGRICISGDSAGGNLAAALGQQFTQDASLKNKLKLQALIYPVLQALDFNTPSYQQNVNTPILPRYVMVKYWVDYFKGNYDFVQAMIVNNHTSLDVEEAAAVRARLNWTSLLPASFTKNYKPVVQTTGNARIVQELPQLLDARSAPLIADQAVLQLLPKTYIMTCEHDVLRDDGIMYAKRLESAGVEVTLDHFEDGFHGCMIFTSWPTNFSVGIRTRNSYIKWLDQNL 相同性解析による交差性が予測される生物種 |
| Full Name | arylacetamide deacetylase-like 1 |
| Calculated molecular weight | 46 kDa |
| Observed molecular weight | 40-50 kDa |
| GenBank accession number | BC047588 |
| Gene Symbol | AADACL1 |
| Gene ID (NCBI) | 57552 |
| RRID | AB_3745693 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q6PIU2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
AADACL1(Arylacetamide deacetylase-like 1) is also named as NCEH1, KIAA1363 and belongs to the 'GDXG' lipolytic enzyme family. The transmembrane enzyme, AADACL1, controls the production of the monoalkylglycerol ether (MAGE) class of NELs in cancer cells and acts as a 2-acetyl MAGE hydrolase and is likely the principal source for this activity in tumor cells(PMID:21513884). The full length protein has three glycosylation sites and can be N-glycosylated(PMID:19159218). It has 3 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for AADACL1 antibody 87679-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |