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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using A2BP1 Polyclonal antibody (22647-1-AP)

    WB analysis using 22647-1-AP (same clone as 22647-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22647-1-AP (A2BP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22647-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using A2BP1 Polyclonal antibody (22647-1-AP)

    IP experiment of mouse brain using 22647-1-AP (same clone as 22647-1-PBS)

    IP result of anti-A2BP1 (IP:22647-1-AP, 4ug; Detection:22647-1-AP 1:500) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 22647-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using A2BP1 Polyclonal antibody (22647-1-AP)

    IHC staining of mouse brain using 22647-1-AP (same clone as 22647-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22647-1-AP (A2BP1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 22647-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using A2BP1 Polyclonal antibody (22647-1-AP)

    IHC staining of mouse brain using 22647-1-AP (same clone as 22647-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22647-1-AP (A2BP1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 22647-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using A2BP1 Polyclonal antibody (22647-1-AP)

    IF Staining of mouse brain using 22647-1-AP (same clone as 22647-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using A2BP1 antibody (22647-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22647-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using A2BP1 Polyclonal antibody (22647-1-AP)

    IF Staining of mouse brain using 22647-1-AP (same clone as 22647-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using A2BP1 antibody (22647-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22647-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using A2BP1 Polyclonal antibody (22647-1-AP)

    IF Staining of mouse brain using 22647-1-AP (same clone as 22647-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using A2BP1 antibody (22647-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22647-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

A2BP1 Polyclonal antibody, PBS Only

A2BP1 Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA
Cat No. 22647-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Synonyms

FOX1, RBFOX1, HRNBP1, Hexaribonucleotide-binding protein 1, Fox-1 homolog A

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22647-1-PBS targets A2BP1 in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag18527

Product name: Recombinant human A2BP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-128 aa of BC113691

Sequence: MLASQGVLLHPYGVPMIVPAAPYLPGLIQGNQEAAAAPDTMAQPYASAQFAPPQNGIPAEYTAPHPHPAPEYTGQTTVPEHTLNLYPPAQTHSEQSPADTSAQTVSGTATQTDDAAPTDGQPQTQPSE

相同性解析による交差性が予測される生物種
Full Name ataxin 2-binding protein 1
Calculated molecular weight 395 aa, 42 kDa
Observed molecular weight 45-70 kDa
GenBank accession numberBC113691
Gene Symbol A2BP1
Gene ID (NCBI) 54715
RRIDAB_2879142
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NWB1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

A2BP1, also named as FOX1 and HRNBP1, contains one RRM (RNA recognition motif) domain. A2BP1 recognizes a (U)GCAUG stretch in regulated exons or in flanking introns. The protein binds to the C-terminus of ataxin-2 and may contribute to the restricted pathology of spinocerebellar ataxia type 2 (SCA2). Ataxin-2 is the product of the SCA2 gene which causes familial neurodegenerative diseases. A2BP1 and ataxin-2 are both localized in the trans-Golgi network. This is a rabbit polyclonal antibody raised against the N terminus of human A2BP1.A2BP1 has two mouse isoforms (A2BP1-A016 and -A030) exogenously expressed in COS7 cells showed a molecular mass of 65 kDa, although the calculated molecular masses of A016 and A030 are 40 and 42 kDa, respectively in the course of cell biological study.

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