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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using A1CF Recombinant antibody (84322-4-RR)

    WB analysis using 84322-4-RR (same clone as 84322-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84322-4-RR (A1CF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84322-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using A1CF Recombinant antibody (84322-4-RR)

    IHC staining of mouse colon using 84322-4-RR (same clone as 84322-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84322-4-RR (A1CF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84322-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using A1CF Recombinant antibody (84322-4-RR)

    IHC staining of mouse colon using 84322-4-RR (same clone as 84322-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84322-4-RR (A1CF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84322-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84322-4-RR

    Affinity and Kinetic Characterization of 84322-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84322-4-RR against Human A1CF were performed. The affinity constant is 0.191 nM.

A1CF Recombinant monoclonal antibody, PBS Only

A1CF Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 84322-4-PBS
Clone No.241644F10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

APOBEC1CF, APOBEC1 complementation factor, ACF65, ACF64, ACF

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84322-4-PBS targets A1CF in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag22929

Product name: Recombinant human A1CF protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-122 aa of BC054873

Sequence: MESNHKSGDGLSGTQKEAALRALVQRTGYSLVQENGQRKYGGPPPGWDAAPPERGCEIFIGKLPRDLFEDELIPLCEKIGKIYEMRMMMDFNGNNRGYAFVTFSNKVEAKNAIKQLNNYEIR

相同性解析による交差性が予測される生物種
Full Name APOBEC1 complementation factor
Calculated molecular weight 594 aa, 65 kDa
Observed molecular weight65 kDa
GenBank accession numberBC054873
Gene Symbol A1CF
Gene ID (NCBI) 29974
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9NQ94
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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