Validation Data Gallery
Tested Applications
| Positive WB detected in | recombinant protein |
| Positive IF/ICC detected in | Transfected HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 125 publications below |
| IHC | See 1 publications below |
| IF | See 14 publications below |
| IP | See 26 publications below |
| ELISA | See 1 publications below |
| CoIP | See 8 publications below |
| ChIP | See 1 publications below |
Product Information
10001-0-AP targets 6*His, His-tag in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with recombinant protein samples.
| Tested Reactivity | recombinant protein |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Recombinant protein 相同性解析による交差性が予測される生物種 |
| Full Name | 6*His, His-tag |
| Calculated molecular weight | 1 kDa |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_11232228 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | HISTAG |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The antibody reacts to the 6*His tagged protein.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for 6*His, His-tag antibody 10001-0-AP | Download protocol |
| WB protocol for 6*His, His-tag antibody 10001-0-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Cell Epithelial cells activate fibroblasts to promote esophageal cancer development | ||
Cell Peripheral cancer attenuates amyloid pathology in Alzheimer's disease via cystatin-c activation of TREM2. | ||
Cell Metab PDIA3 defines a novel subset of adipose macrophages to exacerbate the development of obesity and metabolic disorders | ||
Mol Plant Heat stress-induced decapping of WUSCHEL mRNA enhances stem cell thermotolerance in Arabidopsis |