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Validation Data Gallery

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  • Western Blot (WB) analysis of pig spleen tissue using 5 Lipoxygenase Monoclonal antibody (66326-1-Ig)

    WB analysis of pig spleen using 66326-1-Ig (same clone as 66326-1-PBS)

    pig spleen tissue were subjected to SDS PAGE followed by western blot with 66326-1-Ig (5 Lipoxygenase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66326-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of THP-1 cells using 5 Lipoxygenase Monoclonal antibody (66326-1-Ig)

    WB analysis of THP-1 using 66326-1-Ig (same clone as 66326-1-PBS)

    WB result of 5 Lipoxygenase antibody (66326-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-5 Lipoxygenase transfected THP-1 cells. This data was developed using the same antibody clone with 66326-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of THP-1 cells using 5 Lipoxygenase Monoclonal antibody (66326-1-Ig)

    WB analysis of THP-1 using 66326-1-Ig (same clone as 66326-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 66326-1-Ig (5 Lipoxygenase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66326-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig spleen tissue using 5 Lipoxygenase Monoclonal antibody (66326-1-Ig)

    WB analysis of pig spleen using 66326-1-Ig (same clone as 66326-1-PBS)

    pig spleen tissue were subjected to SDS PAGE followed by western blot with 66326-1-Ig (ALOX5 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66326-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using 5 Lipoxygenase Monoclonal antibody (66326-1-Ig)

    IHC staining of human lung using 66326-1-Ig (same clone as 66326-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66326-1-Ig (ALOX5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66326-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using 5 Lipoxygenase Monoclonal antibody (66326-1-Ig)

    IHC staining of human lung using 66326-1-Ig (same clone as 66326-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66326-1-Ig (ALOX5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66326-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using 5 Lipoxygenase Monoclonal antibody (66326-1-Ig)

    IHC staining of human lung using 66326-1-Ig (same clone as 66326-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66326-1-Ig (ALOX5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66326-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using 5 Lipoxygenase Monoclonal antibody (66326-1-Ig)

    IHC staining of human lung using 66326-1-Ig (same clone as 66326-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66326-1-Ig (ALOX5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66326-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using 5 Lipoxygenase Monoclonal antibody (66326-1-Ig)

    IHC staining of human spleen using 66326-1-Ig (same clone as 66326-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66326-1-Ig (ALOX5 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66326-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using 5 Lipoxygenase Monoclonal antibody (66326-1-Ig)

    IHC staining of human spleen using 66326-1-Ig (same clone as 66326-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66326-1-Ig (ALOX5 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66326-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

5 Lipoxygenase Monoclonal antibody, PBS Only

5 Lipoxygenase Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66326-1-PBS
Clone No.3B1B7

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

WB, IHC, ELISA

Synonyms

ALOX5, 3B1B7, 5 LO, 5 LOX, 5-lipoxygenase

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66326-1-PBS targets 5 Lipoxygenase in WB, IHC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag18168

Product name: Recombinant human ALOX5 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-349 aa of BC130332

Sequence: MPSYTVTVATGSQWFAGTDDYIYLSLVGSAGCSEKHLLDKPFYNDFERGAVDSYDVTVDEELGEIQLVRIEKRKYWLNDDWYLKYITLKTPHGDYIEFPCYRWITGDVEVVLRDGRAKLARDDQIHILKQHRRKELETRQKQYRWMEWNPGFPLSIDAKCHKDLPRDIQFDSEKGVDFVLNYSKAMENLFINRFMHMFQSSWNDFADFEKIFVKISNTISERVMNHWQEDLMFGYQFLNGCNPVLIRRCTELPEKLPVTTEMVECSLERQLSLEQEVQQGNIFIVDFELLDGIDANKTDPCTLQFLAAPICLLYKNLANKIVPIAIQLNQIPGDENPIFLPSDAKYDWL

相同性解析による交差性が予測される生物種
Full Name arachidonate 5-lipoxygenase
Calculated molecular weight 674 aa, 78 kDa
Observed molecular weight 78 kDa
GenBank accession numberBC130332
Gene Symbol 5 Lipoxygenase
Gene ID (NCBI) 240
RRIDAB_2881707
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP09917
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ALOX5(arachidonate 5-lipoxygenase) is also named as LOG5, 5-LO and belongs to the lipoxygenase family. It catalyzes the first step in leukotriene biosynthesis, and plays a role in inflammatory processes. It is a 78 kDa iron-containing enzyme that translocates from the cytosol to associate with FLAP, and converts arachidonate in two steps into the unstable intermediate LTA4. It is essential for cysteinyl-leukotriene (cys-LT) production, critical mediators in asthma(PMID:12911785).

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