Validation Data Gallery
Tested Applications
Recommended dilution
Application | Dilution |
---|---|
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. |
Product Information
68579-1-PBS targets 5-Hydroxymethylcytidine in Dot Blot, ELISA, Indirect ELISA applications and shows reactivity with chemical compound samples.
Tested Reactivity | chemical compound |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | PTG 相同性解析による交差性が予測される生物種 |
Full Name | 5-Hydroxymethylcytidine |
Gene Symbol | |
Gene ID (NCBI) | |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS only , pH 7.3 |
Storage Conditions | Store at -80°C. |
Background Information
Oxidation of 5-methylcytosine in DNA by ten-eleven translocation (Tet) family of enzymes has been demonstrated to play a significant role in epigenetic regulation in mammals. Recent reaearch shows that Tet enzymes also possess the activity of catalyzing the formation of 5-hydroxymethylcytidine (5-hmrC) in RNA. It is known that RNA carries more than 100 distinct types of modifications, and these modifications modulate the structure and functions of RNA. Ribonucleoside 5-methylcytidine (m5C) is subject to oxidative processing in mammals, forming 5-hydroxymethylcytidine (hm5C) and 5-formylcytidine (f5C). Researchers have identified hm5C in total RNA from all three domains of life and in polyA-enriched RNA fractions from mammalian cells. This suggests m5C oxidation is a conserved process that could have critical regulatory functions inside cells (PMID: 25676849).
Protocol for Dot Blot:
https://www.ptglab.com/protocol/68579-1-IgDotBlot.pdf