Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells |
| Positive IHC detected in | Esophagus cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
65030-1-Ig targets 4EBP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Recombinant Protein 相同性解析による交差性が予測される生物種 |
| Full Name | eukaryotic translation initiation factor 4E binding protein 1 |
| Calculated molecular weight | 118 aa, 12 kDa |
| Observed molecular weight | 13 kDa/18-30 kDa |
| GenBank accession number | BC004459 |
| Gene Symbol | EIF4EBP1 |
| Gene ID (NCBI) | 1978 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q13541 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at 2-8°C. |