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Validation Data Gallery

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  • Dot Blot experiment of RNA using 1-Methyladenosine Monoclonal antibody (68636-1-Ig)

    Dot Blot experiment of RNA using 68636-1-Ig (same clone as 68636-1-PBS)

    Total RNA was isolated from HeLa cell line and was dotted to NC membrane at different amount as indicated above the dots. The membrane was blocked with 1% BSA and blotted with m1A (1-Methyladenosine) antibody 68636-1-Ig at 1:2000 followed by incubation of HRP-goat anti-mouse secondary antibody. Signal was developed by ECL substrate. A parallel dot blot was performed using Mouse IgG2a isotype control Monoclonal antibody 66360-2-Ig at the same dose. This data was developed using the same antibody clone with 68636-1-PBS in a different storage buffer formulation.

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Na 1-Methyladenosine Monoclonal antibody, PBS Only

1-Methyladenosine Monoclonal Antibody for Dot Blot, Indirect ELISA
Cat No. 68636-1-PBS
Clone No.2F2H8

Host / Isotype

Mouse / IgG2a

Reactivity

chemical compound

Applications

Dot Blot, Indirect ELISA

Synonyms

N1-methyladenosine, m1A

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68636-1-PBS targets 1-Methyladenosine in Dot Blot, Indirect ELISA applications and shows reactivity with chemical compound samples.

Tested Reactivity chemical compound
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PTG 相同性解析による交差性が予測される生物種
Full Name 1-Methyladenosine
Gene Symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

1-Methyladenosine, also known as m1A, belongs to the class of organic compounds known as purine nucleosides. M1A is important for the tRNA 3-dimensional structure, and it is also important for structure stabilization of tRNA due to its positive electrostatic charge and assists in correct folding of tRNA. Recently evidence suggests that m1A in tRNA is under reversible regulation that is installed by a tRNA m1A methyltransferase (MTase) and is erased by AlkB Homolog 1 (ALKBH1) and AlkB Homolog 3 (ALKBH3).

Protocol for Dot Blot:
https://www.ptglab.com/protocol/68636-1-IgDotBlot.pdf