Recombinant Human SERPING1/C1 Inhibitor protein (rFc Tag)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg9525
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human SERPING1 protein Asn23-Ala500 (Accession# P05155-1) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 710 |
| Accession | P05155-1 |
| PredictedSize | 78.4 kDa |
| SDS-PAGE | 100-135 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
SERPING1, also known as C1 Inhibitor (C1INH), is a member of the Serine proteinase inhibitor family. Its main function is the inhibition of the complement system to prevent spontaneous activation. It inhibits activated C1r and C1s of the first complement component and thus regulates complement activation. Deficiency of this protein is associated with hereditary angioneurotic oedema (HANE).
References:
1. Cugno, Massimo et al. Trends in molecular medicine vol. 15,2 (2009): 69-78. 2. Haslund, Didde et al. The Journal of clinical investigation vol. 129,1 (2019): 388-405. 3. Ryø, Laura Barrett et al. The Journal of allergy and clinical immunology vol. 152,5 (2023): 1218-1236.e9.