Recombinant Mouse RAB5A protein (rFc Tag)
Species
Mouse
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg9093
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Mouse RAB5A protein Met1-Asn215 (Accession# Q9CQD1) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 271457 |
| Accession | Q9CQD1 |
| PredictedSize | 49.2 kDa |
| SDS-PAGE | 48-54 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
RAB5A is a core member of the Rab family within the Ras superfamily of small GTPases and a marker protein for early endosome formation. Localized in the cell membrane and early endosome membrane, RAB5A acts as a molecular switch regulating the docking and fusion of endocytic vesicles, making it a key regulator of the endocytic pathway.
References:
1. Itoh, Takashi et al. Genes to cells : devoted to molecular & cellular mechanisms vol. 11,9 (2006): 1023-37. 2. Barbosa, M D et al. Mammalian genome : official journal of the International Mammalian Genome Society vol. 7,2 (1996): 166-7. 3. Cong, Xiao Xia et al. Cell death and differentiation vol. 27,8 (2020): 2344-2362.