Recombinant Human IgM protein (rFc Tag)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg6742
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human IgM protein Val105-Trp448 (Accession# P01871-2) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 3507 |
| Accession | P01871-2 |
| PredictedSize | 64.3 kDa |
| SDS-PAGE | 65-85 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
Human IgM (Immunoglobulin M) is the largest antibody in the human humoral immune system and exists in two forms: membrane‑bound (mIgM) and secreted (sIgM). Membrane‑bound IgM is a monomer that serves as the antigen‑recognition component of the B‑cell receptor (BCR) expressed on the surface of B cells, mediating B‑cell activation and the initiation of immune responses. Secreted IgM is a pentameric structure (containing a J chain) that is abundantly secreted by plasma cells; it is the earliest antibody produced during a primary immune response and is highly efficient in activating the classical complement pathway, neutralizing pathogens, and promoting agglutination. Elevated serum IgM levels are observed in acute infections, Waldenström’s macroglobulinemia, and other conditions. Auto‑reactive IgM (e.g., rheumatoid factor) participates in the pathogenesis of autoimmune diseases such as rheumatoid arthritis. Membrane‑bound IgM is a target for CAR‑T therapy in B‑cell lymphomas, while secreted IgM serves as a therapeutic target for various immune‑related diseases.
References:
1. Budeus, Bettina et al. Frontiers in immunology vol. 14 (2023): 1308378. 2. Trevijano-Contador, Nuria et al. Infection and immunity vol. 88,4 (2020): e00046-20. 3. Chen, Qu et al. Nature communications vol. 13,1 (2022): 6314. 4. Saltukoglu, Deniz et al. The EMBO journal vol. 42,4 (2023): e112030.