Recombinant Human ROBO1 protein (rFc Tag)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg6518
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human ROBO1 protein Gln26-Pro897 (Accession# Q9Y6N7-1) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 6091 |
| Accession | Q9Y6N7-1 |
| PredictedSize | 121.2 kDa |
| SDS-PAGE | 115-150 kDa , reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
ROBO1 (Roundabout Guidance Receptor 1) is a type I single-pass transmembrane glycoprotein of the immunoglobulin superfamily, serving as the receptor for SLIT1/2 ligands. It mediates axon guidance, neuronal migration, and angiogenesis during development. Dysregulated ROBO1 drives tumor invasion/metastasis in multiple cancers and pathological ocular neovascularization. It is a preclinical therapeutic target; neutralizing antibodies and small molecules blocking SLIT-ROBO1 signaling are under investigation for cancer and eye diseases.
References:
1. Hannula-Jouppi, Katariina et al. PLoS genetics vol. 1,4 (2005): e50. 2. Bhosle, Vikrant K et al. Nature communications vol. 11,1 4112. 17 Aug. 2020.