Recombinant Human WWTR1/TAZ protein (rFc Tag)
Species
Human
Purity
>80 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg6470
Validation Data Gallery
Product Information
| Purity | >80 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human WWTR1 protein Met1-Leu400 (Accession# Q9GZV5) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 25937 |
| Accession | Q9GZV5 |
| PredictedSize | 69.7 kDa |
| SDS-PAGE | 27-38 kDa and 90-120 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
TAZ, also referred as WWTR1, is a transcriptional co-activator with a PDZ-binding motif that is regulated by its interaction with 14-3-3 protein. TAZ is expressed in many primary tumors, such as breast cancer, thyroid carcinoma, colorectal cancer, and glioma. TAZ has been reported to be one of the nuclear effectors of Hippo-related pathways that regulate organ size control, cancer stem cell (CSC) properties, and epithelial-mesenchymal transition (EMT). TAZ has been defined for its role in the nucleus, where it functions directly as a transcriptional regulator by interacting with several nuclear factors as Runx2/Cbfa1, NHERF-2, TEF-1, TBX5, PAX3, PAX8 and TTF-1.
References:
1. Liu, Chenying et al. Int J Biochem Mol Biol. vol. 2,3 (2011): 247-56. 2. Li, Weijie et al. Oncotarget vol. 7,50 (2016): 82686-82699.