Recombinant Rat CD200 protein (rFc Tag)
Species
Rat
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg6121
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Rat CD200 protein Gln31-Gly232 (Accession# P04218) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 24560 |
| Accession | P04218 |
| PredictedSize | 47.9 kDa |
| SDS-PAGE | 55-67 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
CD200, also known as OX2, is a type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily. It contains two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. CD200 is expressed on a variety of cell types, including thymocytes, B lymphocytes, a subset of T lymphocytes, follicular dendritic cells, neurons, and endothelial cells. CD200 binds to its receptor, CD200R, which is primarily expressed by myeloid and T cell lineage. The CD200-CD200R interaction plays a role in immunosuppression and suppression of anti-tumor immune responses.
References:
1. McCaughan, G W et al. Immunogenetics vol. 25,5 (1987): 329-35. 2. Wright, G J et al. Immunity vol. 13,2 (2000): 233-42. 3. Gorczynski, Reginald et al. Journal of immunology (Baltimore, Md. : 1950) vol. 172,12 (2004): 7744-9. 4. Rygiel, T P et al. Oncogene vol. 31,24 (2012): 2979-88.