Recombinant Human PICAL protein (rFc Tag)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg5966
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human PICAL protein Ser2-Met652 (Accession# Q13492-1) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 8301 |
| Accession | Q13492-1 |
| PredictedSize | 96.2 kDa |
| SDS-PAGE | 100-140 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
PICALM (Phosphatidyl Inositol Clathrin Assembly Lymphoid Myeloid protein) is a ubiquitously expressed protein that plays a role in clathrin-mediated endocytosis. PICALM also affects the internalization and trafficking of SNAREs and modulates macroautophagy. PICALM is a highly validated genetic risk factor for Alzheimer's disease (AD). The long isoform of human PICALM has 22 exons (encoding PICALML), while the short isoform lacks exon 14 (encoding PICALMS).
References:
1.Zhang, Feifei et al. Molecular cancer vol. 20,1 6. 4 Jan. 2021. 2.Mercer, Jacob L et al. PloS one vol. 10,6 e0129776. 15 Jun. 2015. 3.Zhao, Zhen et al. Nat Neurosci. 2015;18(7):978-987.