Recombinant Human Alpha Synuclein protein (rFc Tag)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg5390
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human Alpha Synuclein protein Met1-Ala140 (Accession# P37840-1) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 6622 |
| Accession | P37840-1 |
| PredictedSize | 40.5 kDa |
| SDS-PAGE | 10 kDa and 35-68 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
Alpha Synuclein (α-syn) is a phosphoprotein that is primarily localize to the presynaptic terminals of mature neurons, where it is involved in synaptic function and plasticity. Α-syn has drawn intense interest ever since the late 1990s, when the first α-synuclein missense mutation was identified as a cause of familial Parkinson's disease (PD). Aggregated and hyper-phosphorylated forms of α-syn protein are the pathological hallmark of Lewy body disease, which includes Parkinson's disease (PD), diffuse Lewy body disease (DLBD), and Lewy body variant of Alzheimer's disease (LBV). This antibody can recognize all the isoforms of α-syn.
References:
1. Spillantini, M G et al. Nature vol. 388,6645 (1997): 839-40. 2. Choi, Insup et al. Nature communications vol. 11,1 1386. 13 Mar. 2020. 3. Singleton, A B et al. Science (New York, N.Y.) vol. 302,5646 (2003): 841.