Recombinant Human P-selectin/CD62P protein ( rFc Tag)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg5366
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human P-selectin protein Thr380-Ala771 (Accession# P16109) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 6403 |
| Accession | P16109 |
| PredictedSize | 67.5 kDa |
| SDS-PAGE | 77-100 kDa , reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
P-selectin, also known as GMP140 or CD62P, is a transmembrane glycoprotein that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is an adhesion molecule involved in the pathogenesis of inflammation, thrombosis, and oncogenesis. P-selectin is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.
References:
1. André, Patrick. British journal of haematology vol. 126,3 (2004): 298-306. 2. Purdy, Megan et al. Journal of thrombosis and haemostasis : JTH vol. 20,5 (2022): 1056-1066. 3. Ling, Tingting et al. Medical oncology (Northwood, London, England) vol. 40,11 338. 23 Oct. 2023.