Recombinant Human CRH/CRF protein (rFc Tag)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg4974
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human CRH protein Leu25-Ile194 (Accession# P06850) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 1392 |
| Accession | P06850 |
| PredictedSize | 44.8 kDa |
| SDS-PAGE | 43-50 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in 0.5M arg，30mM Tris，pH8.0. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
CRH, also called CRF or corticoliberin, is a peptide hormone and neurotransmitter involved in the stress response. Marked reduction in this protein has been observed in association with Alzheimer disease. In addition to production in the hypothalamus, this protein is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. In the placenta it is a marker that determines the length of gestation and the timing of parturition and delivery.
References:
1. Makrigiannakis A, et al. (2018) Curr Mol Pharmacol. 11(1):81-87. 2. Zhu C, Li S. (2024) Front Endocrinol (Lausanne). 15:1363748. 3. Bamberger CM, Bamberger AM. (2000) Ann N Y Acad Sci. 917:290-296. 4. Rassouli O, et al. (2018) Curr Mol Pharmacol. 11(1):72-80.