Recombinant Human CD73 protein (rFc Tag)
Species
Human
Purity
>85 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg2974
Validation Data Gallery
Product Information
| Purity | >85 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human CD73 protein Trp27-Lys547 (Accession# P21589-1) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 4907 |
| Accession | P21589-1 |
| PredictedSize | 83.8 kDa |
| SDS-PAGE | 80-95 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
CD73, also known as ecto-5'-nucleotidase (5'-NT), is a 70-kDa, glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues . CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates. In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells. CD73 is highly expressed in many human solid tumors and is closely involved in cancer progression .
References:
1.Colgan, Sean P et al. Purinergic signalling vol. 2,2 (2006): 351-60. 2.Jin, Dachuan et al. Cancer research vol. 70,6 (2010): 2245-55. 3.Resta, R et al. Immunological reviews vol. 161, (1998): 95-109. 4.Thomson, L F et al. Tissue antigens vol. 35,1 (1990): 9-19. 5.Jin, Dachuan et al. Cancer research vol. 70,6 (2010): 2245-55.
