Recombinant Mouse IGFBP1 protein (rFc Tag)(HPLC verified)
Species
Mouse
Purity
>90 %, SDS-PAGE
>90 %, SEC-HPLC
Tag
rFc Tag
Activity
not tested
Cat no : Eg2934
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE >90 %, SEC-HPLC |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Mouse IGFBP1 protein Ala26-Asn272 (Accession# P47876) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 16006 |
| Accession | P47876 |
| PredictedSize | 52.9 kDa |
| SDS-PAGE | 52-60 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
IGFBP1 is a binding protein that is produced primarily by the liver outside of pregnancy and is highly expressed by the placenta. IGFBP1 has been implicated in the modulation of the biological activity of insulin-like growth factor (IGF)-1 and IGF-2, which are key regulators of growth and metabolism in postnatal and fetal life.
References:
1. Uhlén M, et al. (2015). Science. ;347:1260419. 2. Duan C, et al. (2005). Comp. Endocrinol. ;142:44–52.

