Recombinant Human IL-25/IL-17E protein (rFc Tag)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg2803
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human IL-25 protein Tyr33-Gly177 (Accession# Q9H293-1) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 64806 |
| Accession | Q9H293-1 |
| PredictedSize | 42.3 kDa |
| SDS-PAGE | 43-55 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
Interleukin-25 (IL-25), also known as IL-17E, is a unique member of the interleukin-17 (IL-17) cytokine family. IL-25 is a key orchestrator of type 2 immune responses, which are central to host defense against helminths, the pathogenesis of allergic disorders, and tissue repair. Its expression is not confined to classical immune cells but is notably abundant in various epithelial cell lineages, positioning it as a critical sentinel at mucosal barriers. IL-25 is critically involved in allergic disorders such as asthma, allergic rhinitis, and chronic rhinosinusitis with nasal polyps (CRSwNP). Its levels in induced sputum correlate with disease severity, eosinophil counts, and lung function in asthmatic children. IL-25 exhibits a dual and complex role in oncology. In colorectal cancer (CRC), its expression correlates with poor survival. Conversely, in breast cancer, serum IL-25 levels are lower in patients compared to healthy subjects, and reduced IL-25 is associated with higher tumor grade and stage.
References:
1.Yuan, Qingfang et al. Biomarker research vol. 11,1 (2023): 36. 2.Ji, Ming et al. Nature vol. 529,7585 (2016): 221-5. 3.Abdu, Semah et al. Allergy vol. 80,4 (2025): 965-975. 4.Zhao, Zewei et al. Frontiers in immunology vol. 13, (2022): 837262. 5.Shokrollahi Barough, Mahdieh et al. J Interferon Cytokine Res vol. 40,3 (2020): 139-144.