Recombinant Human CD109 protein (rFc Tag)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg2673
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human CD109 protein Val22-Ser1268 (Accession# Q6YHK3-1, Tyr703Ser and Tyr1241Met) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 135228 |
| Accession | Q6YHK3-1 |
| PredictedSize | 165.2 kDa |
| SDS-PAGE | 135-220 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
CD109, also named as CPAMD7, is a member of the alpha2-macroglobulin/complement superfamily and is preferentially expressed on hematopoietic stem as well as progenitor cell subsets. It is a glycosylphosphatidylinositol (GPI)-anchored protein, first identified as a marker for activated T cells and platelets. CD109 stands out as the most potent negative regulator of canonical TGF-β signaling and responses. Additionally, CD109 might be a useful molecular target for the development of new therapeutics for malignant tumors, such as squamous cell carcinoma.
References:
1. Lin, Martin et al. Blood vol. 99,5 (2002): 1683-91. 2. Murray, L J et al. Experimental hematology vol. 27,8 (1999): 1282-94. 3. Sutherland, D R et al. Blood vol. 77,1 (1991): 84-93. 4. Tam, B Y et al. Journal of cellular biochemistry vol. 70,4 (1998): 573-86. 5. Tam, B Y et al. Journal of cellular biochemistry vol. 83,3 (2001): 494-507. 6. Hashimoto, Mizuo et al. Oncogene vol. 23,20 (2004): 3716-20.