Recombinant Mouse MAdCAM-1 protein (rFc Tag)
Species
Mouse
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg2010
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Mouse MAdCAM-1 protein Gln22-Ser364 (Accession# NP_038619) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 17123 |
| Accession | NP_038619 |
| PredictedSize | 63.1 kDa |
| SDS-PAGE | 68-90kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
Lymphocyte recirculation through different organs is thought to be regulated by adhesion molecules ("homing receptors") recognizing tissue-specific vascular addressins on endothelium. The mucosal vascular addressin, MAdCAM1, is an immunoglobulin superfamily adhesion receptor for lymphocytes that is expressed by mucosal venules and helps direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. MAdCAM1 can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes.
References:
1. Hamann, A et al. Journal of immunology (Baltimore, Md. : 1950) vol. 152,7 (1994): 3282-93. 2. Berlin, C et al. Cell vol. 74,1 (1993): 185-95. 3. Fu, Shan et al. Cellular and molecular gastroenterology and hepatology vol. 17,2 (2024): 199-217.