Recombinant Mouse CD19 protein (His Tag)
Species
Mouse
Purity
>70 %, SDS-PAGE
Tag
His Tag
Activity
not tested
Cat no : Eg0907
Validation Data Gallery
Product Information
| Purity | >70 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Mouse CD19 protein Arg19-Gly287 (Accession# P25918) with a His tag at the C-terminus. |
| GeneID | 12478 |
| Accession | P25918 |
| PredictedSize | 31.2 kDa |
| SDS-PAGE | 42-55 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
CD19 is a type I transmembrane glycoprotein belonging to the immunoglobulin superfamily. It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells. CD19 is also involved in B cell development, activation, and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction.
References:
1. Tedder, T F, and C M Isaacs. Journal of immunology (Baltimore, Md. : 1950) vol. 143,2 (1989): 712-7. 2. Scheuermann, R H, and E Racila. Leukemia & lymphoma vol. 18,5-6 (1995): 385-97. 3. Wang, Kemeng et al. Experimental hematology & oncology vol. 1,1 (2012): 36.