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CoraLite® Plus 647 Anti-Mouse CD134 (OX-86)

CD134 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG1, kappa

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

OX-86

Cat no : CL647-65136

Synonyms

ACT35, CD134, Ly 70, Ox40, Tnfrsf4, Txgp1, TXGP1L

Validation Data Gallery

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  • Flow cytometry (FC) experiment of BALB/c mouse splenocytes using CoraLite® Plus 647 Anti-Mouse CD134 (OX-86) (CL647-65136)

    FC experiment of BALB/c mouse splenocytes using CL647-65136

    1X10^6 unstimulated or Con A-stimulated (3 ug/ml, 2 days) BALB/c mouse splenocytes were surface stained with CoraLite®488 Anti-Mouse CD3 (CL488-65077, Clone: 17A2) and 0.25 ug CoraLite® Plus 647 Anti-Mouse CD134 (CL647-65136, Clone: OX-86). Cells were not fixed.

Tested Applications

Positive FC detected inCon A-stimulated mouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65136 targets CD134 in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen N/A
Full Name tumor necrosis factor receptor superfamily, member 4
GenBank Accession NumberBC065782
Gene Symbol Tnfrsf4
Gene ID (NCBI) 22163
RRIDAB_2920262
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD134, also known as OX40 and TNFRSF4, is a member of the TNFR-superfamily of receptors (PMID: 2828930; 9766631). It is a type I transmembrane protein predominantly expressed on activated T cells which include CD4 and CD8 T cells, Th2, Th1, and Th17 cells, as well as regulatory T cells (Tregs) (PMID: 20307208). CD134 is activated by its cognate ligand CD134L (OX40L) and functions as a T cell co-stimulatory molecule (PMID: 26215166). CD134-CD134L interactions have been proposed as a potential therapeutic target for treating autoimmune diseases, cancer and infectious disease (PMID: 26215166; 19426222).

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 CD134 antibody CL647-65136Download protocol
Standard Protocols
Click here to view our Standard Protocols