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CoraLite®555-conjugated TFAP2C Polyclonal antibody

TFAP2C Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

Cat no : CL555-14572

Synonyms

ap 2gamma, AP2 GAMMA, ERF1, hAP 2g, TFAP2C, TFAP2G, Transcription factor ERF 1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of MCF-7 cells using CoraLite®555-conjugated TFAP2C Polyclonal antibody (CL555-14572)

    FC experiment of MCF-7 using CL555-14572

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human TFAP2C (CL555-14572) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-14572 targets TFAP2C in FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TFAP2C fusion protein Ag5648
Full Name transcription factor AP-2 gamma (activating enhancer binding protein 2 gamma)
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC035664
Gene Symbol TFAP2C
Gene ID (NCBI) 7022
RRIDAB_2919618
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
FC protocol for CL555 TFAP2C antibody CL555-14572Download protocol
Standard Protocols
Click here to view our Standard Protocols