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CoraLite® Plus 488-conjugated VISTA Polyclonal antibody

VISTA Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-24849

Synonyms

B7-H5, C10orf54, GI24, Platelet receptor Gi24, PP2135, SISP1, UNQ730/PRO1412, VISTA, VSIR

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using CoraLite® Plus 488-conjugated VISTA Polyclonal ant (CL488-24849)

    IF Staining of human placenta using CL488-24849

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CoraLite® Plus 488 VISTA antibody (CL488-24849) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using CoraLite® Plus 488-conjugated VISTA Polyclonal ant (CL488-24849)

    IF Staining of human placenta using CL488-24849

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CoraLite® Plus 488 VISTA antibody (CL488-24849) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IF detected inhuman placenta tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-24849 targets VISTA in IF applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VISTA fusion protein Ag20600
Full Name chromosome 10 open reading frame 54
Calculated Molecular Weight 311 aa, 34 kDa
Observed Molecular Weight45-60 kDa
GenBank Accession NumberBC020568
Gene Symbol C10orf54
Gene ID (NCBI) 64115
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VISTA, also known as platelet receptor Gi24, B7-H5, C10orf54, Dies1, PD-1H and SISP1, is a member of the immunoglobulin superfamily. It serves as not only as a positive regulator but also as a substrate of MT1-MMP, and contributes at least in part to tumor invasion.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 VISTA antibody CL488-24849Download protocol
Standard Protocols
Click here to view our Standard Protocols