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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated INF2 Polyclonal antibody

INF2 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-20466

Synonyms

C14orf151, C14orf173, DKFZp762A0214, FLJ22056, HBEBP2 binding protein C, INF2, Inverted formin 2, pp9484

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated INF2 Polyclonal anti (CL488-20466)

    IF Staining of HepG2 using CL488-20466

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 INF2 antibody (CL488-20466) at dilution of 1:200. DAPI (blue).

Tested Applications

Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-20466 is INF2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen INF2 fusion protein Ag14302
Full Name inverted formin, FH2 and WH2 domain containing
Calculated Molecular Weight 1249 aa, 136 kDa
Observed Molecular Weight 180-200 kDa
GenBank Accession NumberBC008756
Gene Symbol INF2
Gene ID (NCBI) 64423
RRIDAB_2919171
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

INF2 is a member of the formin family of actin-regulating proteins. INF2 is peripherally bound to the cytoplasmic face of the endoplasmic reticulum (ER). Mutations in the formin gene INF2 cause focal segmental glomerulosclerosis. The predicted MW of INF2 is around 136 kDa, while higher molecular mass from 140-200 kDa has been reported in literature (26383224,20493814,19366733).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 INF2 antibody CL488-20466Download protocol
Standard Protocols
Click here to view our Standard Protocols