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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated HNRNPUL1 Polyclonal antibody

HNRNPUL1 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10578

Synonyms

E1B AP5, E1BAP5, FLJ12944, HNRNPUL1, HNRPUL1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated HNRNPUL1 Polyclonal (CL488-10578)

    IF Staining of HepG2 using CL488-10578

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 HNRNPUL1 antibody (CL488-10578) at dilution of 1:200.

Tested Applications

Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-10578 targets HNRNPUL1 in IF applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HNRNPUL1 fusion protein Ag0899
Full Name heterogeneous nuclear ribonucleoprotein U-like 1
Calculated Molecular Weight 120 kDa
Observed Molecular Weight 90-115 kDa
GenBank Accession NumberBC002564
Gene Symbol HNRNPUL1
Gene ID (NCBI) 11100
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HNRNPUL1, also named as E1BAP5, HNRPUL1 and E1B-AP5, acts as a basic transcriptional regulator. It represses basic transcription driven by several virus and cellular promoters. HNRNPUL1 plays also a role in mRNA processing and transport. It is a cellular protein that interacts with the adenovirus protein E1B-55 and implicated in mRNA export. HNRNPUL1 is an hnRNP-like protein that likely mediates the interaction of NXF1 with mRNAs.(PMID:17267598). It may modulate PAN expression in complex with KSHV ORF57(PMID:22043172). HNRNPUL1 specifically bound to the Rae1-Nup98 complex independently of M protein, and this interaction was found to be RNA dependent, as it was greatly diminished in the presence of RNase.(PMID:19745842) HNRNPUL1 has 5 isoforms with calculated MW 84-95kd and 42kd(isoform 5). This antibody can recognize isoform 1-4 .

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 HNRNPUL1 antibody CL488-10578Download protocol
Standard Protocols
Click here to view our Standard Protocols